Subject: | |
From: | |
Reply To: | |
Date: | Tue, 23 Oct 2012 17:08:52 +0000 |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
There are references on the Huygens site about moderate improvement of resolution
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Christophe Leterrier
Sent: Tuesday, October 23, 2012 12:28 PM
To: [log in to unmask]
Subject: Question about deconvolution
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Hi folks,
I have a long-standing question regarding deconvolution (as processing
widefield or confocal images to reassign light from where it originated
using a PSF).
Is there a theoretical limit to the resolution one could obtain using
deconvolution? Is is theoretically possible to "break" the diffraction
limit with deconvolution? That is, to get under the classical 200x200x600nm
spot? I think it is not the case, but then why would you deconvolve
widefield or confocal images? What do you gain by doing so on a system that
is reasonably close to its theoretical capabilities in terms of optical
performances?
Thanks for your help,
Christophe
--
Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France
|
|
|