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October 2012

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From:
"MODEL, MICHAEL" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 23 Oct 2012 17:08:52 +0000
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There are references on the Huygens site about moderate improvement of resolution

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Christophe Leterrier
Sent: Tuesday, October 23, 2012 12:28 PM
To: [log in to unmask]
Subject: Question about deconvolution

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Hi folks,

I have a long-standing question regarding deconvolution (as processing
widefield or confocal images to reassign light from where it originated
using a PSF).

Is there a theoretical limit to the resolution one could obtain using
deconvolution? Is is theoretically possible to "break" the diffraction
limit with deconvolution? That is, to get under the classical 200x200x600nm
spot? I think it is not the case, but then why would you deconvolve
widefield or confocal images? What do you gain by doing so on a system that
is reasonably close to its theoretical capabilities in terms of optical
performances?

Thanks for your help,

Christophe

--
Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France

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