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Date: | Fri, 7 May 1999 11:49:15 +1200 |
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To whom it may concern ,
I am not sure what you are doing with your images but we save ours on the
Leica system as Tiffs and we do not get a stack of black and white images.
You need to merge the separate channels before you save them, then you will
get a stack of overlayed coloured images which can easily be opened in most
imaging software.
At 06:21 PM 5/5/99 -0500, you wrote:
>>Part Two of the problem is that my institution recently bought a Leica
>>confocal, and while the primary data are quite nice the file format seems
>>idiosyncratic.
>
>If you save the data on the leica as Tiff image stacks, what you get is a
>stack of 8bit b/w images: first all the images of the first channel, then
>the second channel etc. You can easily open this e.g. in NIH Image. The LUT
>is saved somewhere in the header. So what you need is a software that can
>merge the "independent" colors into a 24 RGB stack. Back to square one.
>I've heard that IP Lab can do this on the Mac, but I have no own experience.
>
>Steffen
>
>
>-- ------------------------------------
>Dr. Steffen Dietzel
>Dept. of Cell and Structural Biology
>University of Illinois at Urbana-Champaign
>
>B107 Chemical and Life Science Building
>601 S. Goodwin Ave.
>Urbana, IL 61801
>
>Phone: +1/217/333-8372
>Fax: +1/217/244-1648
>
>www: http://www.life.uiuc.edu/belmont/dietzel
>
>
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