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Hi, there -
Is anyone out there imaging Brainbow specimens (we are planning to have
cleared mice brains soon....) with the Zeiss Lightsheet Z1? Our filter
choices do not seem to be ideal for it and we are looking to see what
filters would be recommended and if we can get them in place ASAP. Filter
info below.
Thanks so much!
Available Lasers: 405, 445, 488, 514, 561, and 640nm
Laser blocking filters (these cannot be changed during an experiment,
ideally):
- 405/488/561/640
- 445/514/561
Emission Filters (again, we can use either FW1 or FW2, but cannot really
change during an experiment... at least, not easily... ):
> FW1:
> - LP510, BP 420 - 470, BP 575 - 615
> - LP 510, BP 460-500, BP 525 - 565
> - LP560, BP 505 - 545, BP 575-615
> - LP560, BP 505 - 545, LP 585
> - LP 560, BP 505 - 545, LP 660
> FW2:
> - BP 460 - 500, LP 525 - 565
> - BP 460 - 500, LP 585
> - BP 525 - 565, LP 585
Cheers,
Holly
*Holly Aaron, [log in to unmask] <[log in to unmask]>*
*CRL Molecular Imaging Center*
Register now for AIM:
*Advanced Imaging Methods Workshop*
*January 24 – 26, 2018*
*https://www.regonline.com/AIM2018* <https://www.regonline.com/AIM2018>
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