*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Dear Zulin Yu,
I have seen Visitron efficiently using 2 sCMOS cameras pco.edge with their new yokogawa Spinning disk system.
In general the calculations suggest that from, let's say 10-12 photons the SNR of corresponding sCMOS cameras is better than the emCCD cameras, but this also means front illuminated compared to front illuminated image sensors. From the guys at Visitron I have heard, that they are happy with the synchronization of the sCMOS cameras, but they mostly used Photometrics Evolve cameras back illuminated emCCD cameras before.
Still, if you in the photon counting range, the BI emCCD would do better.
I have to state, that I work for PCO, but I conducted the sCMOS project on our side.
with best regards,
Gerhard Holst
_______________________________
Dr. Gerhard Holst
Science & Research
PCO AG
Donaupark 11
93309 Kelheim, Germany
fon +49 (0)9441 2005 36
fax +49 (0)9441 2005 20
mob +49 (0)172 711 6049
[log in to unmask]
www.pco.de
-----Ursprüngliche Nachricht-----
Von: Confocal Microscopy List [mailto:[log in to unmask]] Im Auftrag von Zulin Yu
Gesendet: Mittwoch, 16. Januar 2013 05:46
An: [log in to unmask]
Betreff: seeking suggestions on new sCMOS camera
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Dear All,
I am writing to ask your suggestions about sCMOS camera on spinning disk
system. Recently, I got a demo (Hamamatsu Flash 4.0) camera on our spinning
disk system (PerkinElmer Ultraview) and I am a little bit disappointed about its
performance - it is a decent camera, but not as good as I expected.
Based on the company's brochure, Flash 4.0 SNR exceeds that of EM-CCDs at
about 6 photon/pixel
(http://sales.hamamatsu.com/assets/pdf/hpspdf/e_flash4.pdf); several research
paper also claimed that the latest sCMOS is as good as EMCCD with more than 15
photons (http://www.ncbi.nlm.nih.gov/pubmed?term=EMCCD%20sCMOS).
However, we still got a much higher SNR (double) on EMCCD even we have more
than 50 photons/pixel. Did anybody have experience with the high end sCMOS
camera and compared it with back-thinned EMCCD? I appreciate any input, Zulin
|
