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Fixation with cold fixatives usually depolimeryze the actin filaments. I
usually fix the cells with room temperature fixative.

Prof. Dr. Francisco Javier Hernandez Blazquez
Universidade de São Paulo
Fac. de Medicina Veterinária e Zootecnia
Departamento de Cirurgia - Setor de Anatomia
Av. Prof. Dr. Orlando Marques de Paiva, 87
05508-000 - São Paulo (SP) - Brasil
Tel..55 (11) 3091 1374  Fax  55 (11) 3091 7805
email: [log in to unmask]

----- Original Message -----
From: "Marylou Zuzarte" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Tuesday, November 11, 2003 7:47 AM
Subject: fixation of myocytes


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I would like some opinion over the fixation of rat heart myocytes. I have
> tried 4% PFA which gave a very high autoflourescence and 50% ea. MeOH-
> Acetone(ice cold). This method gave me  good ( already published) pictures
> of ion channels in the t-tubules.
> What I got was confusing results is when I stained the cells for actin
> using phalloidin.
> I would greatly appreciate some feedback
>