There are several Syto dyes (Molecular Probes) that fluoresce at longer
wavelengths than does GFP.  These are presumably translocated across
functional membranes.  We are not in the eukaryotic business, but we
routinely use Syto 59 to stain bacterial cells that also express GFP and
visualize both emission wavelengths simultaneously.

>Does anyone know of a nuclear stain that can be used with GFP and live
>cells?  We have a Bio-Rad MRC1024 with a Krypton/Argon laser (488nm, 568nm,
>647nm).
>
>Thanks,
>Susan Garfield
>Confocal Facility Manager
>Laboratory of Experimental Carcinogenesis
>Division of Basic Sciences
>National Cancer Institute, NIH
>Building 37, Room 3C28
>37 Convent Drive MSC4255
>Bethesda, MD  20892-4255
>+1 (301)496-5688 x227 (voice); +1 (301)496-0734 (fax)

Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396