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I can't see how PBS can contribute any fluorescence at all.  Neither 
phosphate nor NaCl should be fluorescent at all, and I've routinely used 
phosphate buffers for fluorimetry measurements.

Kurt

Guillermo Palchik wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Confocalists,
>
> I have been trying to locate a paper I had read a few years ago 
> regarding the use of TBS rather than PBS for IHC, since it reduces the 
> level of background fluorescence associated with the PBS. Does anybody 
> know what paper I am referring to?
> In addition, does anybody have any insight into whether this is 
> actually true? I mean, if I do my IHC with PBS and then wash it off 
> with dH20, isn't this getting rid of most of the PBS background 
> fluorescence?
>
> Thanks,
>
> Gil Palchik
>
> [log in to unmask]
>


-- 
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

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