Our chambers have originally been developed for work with volatile 
anesthetics and are made of stainless steel and glass. Two Viton 
O-rings (can be replace by silicone, if you like) are used to seal 
the chamber but the exposed surface is minimized, so even if the 
Viton or silicone should only be partially compatible with your 
solvents, the chamber should still work fine. You can even coat the 
O-rings with PTFE, if required. The perfusion in-/outlets are made of 
steel too, and interface directly to PTFE tubing.

This is another shameless commercial plug, of course :-)

Beat

At 19:25 10-06-2009, you wrote:
>  I've been working lately on confocal microscopy with very volatile 
> and toxic organics.  The chambers described today are quite useful 
> (Matek...) for cell culture and cell imaging; however, the solvents 
> I've been working with lately readily attack plastic and rubber.  I 
> would need a chamber made of glass or fused silica, with a 
> thickness at the bottom comparable to a 0, 1, or 1.5 standard 
> microscope coverslip for an inverted microscope, and Luer Locks or 
> similar pipette access ports made of Teflon or with Teflon 
> plugs.  I haven't found a supplier yet, if any vendor here or any 
> of you know of a vendor that makes any type of chamber with glass 
> only and with PTFE plugs that would be terrific.
>
>Best,
>Evangelos
>Harvard CNS