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Is the difference perhaps according to whether you have a service
contract or not? We have 3 Coherent MP lasers and like Brian, have
always had excellent and fast service. I recently took one of them off
service contract because it is hardly ever used at the moment, so we can
afford downtime more than we can afford the cost of the service
contract, but I am fully aware that if it goes wrong it will now mean a
lengthy and expensive repair back in Scotland.
Jim, was your laser under service contract?
Best,
Alison
On 6/18/2014 5:56 PM, Armstrong, Brian wrote:
> *****
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> *****
>
> Hi All, our experience with Coherent is quite the opposite. When our Chameleon Ultra was failing after 2500hrs, Coherent shipped the new LASER and we swapped them so that we did not lose even a single day of function. I think that we have done this three times now. Each time it has been a smooth transition. Several years ago they replaced a Chameleon 210 with an Ultra without charge. I would buy only Coherent for this very reason.
>
> With many products the service seems to vary depending upon the area and service personnel.
> Our experiences may be confined to Southern California but I believe Coherent company policies are customer oriented.
>
> Cheers,
>
> Brian D Armstrong PhD
> Associate Research Professor
> Director, Light Microscopy Core
> Beckman Research Institute
> City of Hope
> Dept of Neuroscience
> 1450 E Duarte Rd
> Duarte, CA 91010
> 626-256-4673 x62872
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Henthorn, Jim C. (HSC)
> Sent: Wednesday, June 18, 2014 7:34 AM
> To: [log in to unmask]
> Subject: Re: comparison of lasers for MPM
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Pam,
>
> I defiantly would not consider Coherent, since we have a Chameleon XR that died with 334 head hours and our only option is to send it to Scotland for repair for $35,000. I was told that the service with the Mai Tai is much better.
>
> Good luck,
>
> Jim Henthorn
> Flow and Image Cytometry Lab
> 975 NE 10th Street BRC 1317
> Stanton L Young Biomedical Research Building
> Oklahoma City, OK 73104
> [log in to unmask]<mailto:[log in to unmask]>
> (405)-271-2035
> http://research.ouhsc.edu/core-facilities/
>
>
>
>
> On Jun 18, 2014, at 2:50 AM, Sylvie Le Guyader <[log in to unmask]<mailto:[log in to unmask]>> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> *****
>
> Hi Pam
>
> If you are into second and third harmonic generation, it can be useful to design the system so that you can split your TiSa before you pump it to higher wavelength. This way you get one line at 900-1000 nm to excite your fluorophore and one line at 1200-1300 for THG to visualize the tissue structure.
> Both SP DeepSee and Coherent TiSa/OPO can be split that way but the SP DeepSee delivers a fixed lower wavelength (1040nm which works for RFPs) and a tunable longer wavelength (690-1300nm) whereas in the Coherent system, both wavelength can be tuned (more flexible but more expensive).
> The question is then very much if you want to image second and third harmonics.
>
> If you do not need to THG, my understanding is that the SP and Coherent lasers are equivalent although I have only played with the Coherent Ultra II and the question is then if you want some compensation or not as Craig mentioned.
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader
> Live Cell Imaging Unit Manager
> Dept of Biosciences and Nutrition
> Karolinska Institutet
> Hälsovägen 7
> Novum, G lift, floor 6
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> Sweden
> office: +46 (0) 8 5248 1107
> LCI room 1: +46 (0) 8 5248 1172
> LCI room 2: +46 (0) 8 5248 3542
> mobile: +46 (0) 73 733 5008
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Pamela Young
> Sent: 18 June 2014 08:48
> To: [log in to unmask]
> Subject: Re: comparison of lasers for MPM
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v1/url?u=http://lists.umn.edu/cgi-bin/wa?A0%3Dconfocalmicroscopy&k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0A&r=svlHKECXtjjwqCHo3w0sSGcQrYKZauYVxlhKApjyKRQ%3D%0A&m=tRcSo2WLH62w4Fv3gfoe6werNhQ3Y3ZW3f%2BDPIJEvE8%3D%0A&s=fc183722604d7fe60506cf9250269115d1070ad5350ff06a79a944fd50e6de56
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> *****
>
> Excellent point, Craig! I¹m running a core facility, so I have a lot of different users
> with different applications. So I guess from that standpoint, I¹m looking for
> thoughts on how the systems compare in range of use, ease of use, and reliability.
> Because you are right, each user will have a different application!
>
> Dr Pamela A. Young
> | Light and Optical Microscopist
> Australian Centre for Microscopy & Microanalysis
>
> THE UNIVERSITY OF SYDNEY
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> On 18/06/2014 12:55 pm, "Craig Brideau" <[log in to unmask]> wrote:
>
> *****
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> *****
>
> You are asking a bit of an 'apples vs. oranges' question here, in that
> different lasers with different accessories achieve different functions.
> Different lasers will be appropriate or inappropriate, depending on the
> type of imaging you want to do and the types of fluorophores you want
> to work with.
> I always start by asking the user what non-linear imaging they want to do.
> The usual answer is 2-photon, but some also want second harmonic
> generation capability (SHG), and some want higher-order 3-photon
> imaging, although this is pretty rare. This question gives clues as to
> what pulse width and tuning range the user may require.
> The next is what sort of tissues the user wants to image, and how deep
> they want to go. If they want to go very deep, this indicates that
> longer wavelength tuning ranges are appropriate, as well as dispersion
> control with shorter pulse widths, pointing to OPO or just a
> long-tuning Ti:Saph and pulse compression accessories. For relatively
> shallower imaging on not particularly scattering samples, these
> measures are not necessary.
> Then I ask what sort of fluorophores the user is used to working with,
> and which ones they plan to use. This will help nail down exactly what
> excitation wavelengths will be necessary, indicating what sort of
> tuning range will be necessary out of the laser, and whether or not an
> OPO will be needed. For multiple fluorophores it is important to
> determine if all of them can reasonably be excited by a single
> wavelength, or whether a second wavelength would be needed, which again
> points to an OPO for this situation. If the dyes the user wants will
> all work adequately with a single wavelength than just a basic laser is
> sufficient.
> Finally, the experience level of the user, and whether or not the
> system will be a 'core' system for multiple users, influences how
> user-friendly and turnkey the system and its accessories need to be.
> These are not the only considerations, but I hope it gives you some
> idea of the thought processes that go towards selecting a laser.
>
> Craig Brideau
>
>
> On Tue, Jun 17, 2014 at 8:31 PM, Pamela Young
> <[log in to unmask]>
> wrote:
>
> *****
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> *****
>
> Hello List,
>
> Has anyone done any comparisons of MPM lasers? Most of my experience
> has been with various versions of the MaiTai and the InSight DeepSee
> (and of course many much older lasers). So if you have thoughts on
> how these systems compare to the Chameleon and OPO, I would love your
> thoughts.
>
> Thanks,
> Pam
>
> Dr Pamela A. Young | Light and Optical Microscopist Australian Centre
> for Microscopy & Microanalysis
>
> THE UNIVERSITY OF SYDNEY
> Rm 116A, Madsen Building F09 | The University of Sydney | NSW | 2006
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