Hello Nicholas,

We have developed something we call IMS, Intensity modulated Multiple
wavelength Scanning. Several laser beams are modulated and fed into a
CSLM. The detection is using lock-in technique. See the references
below.

     K. Carlsson, N. Åslund, K. Mossberg & J. Philip, "Simultaneous
     confocal recording of multiple fluorescent labels with improved
     channel separation," J. Microsc., 176, 287-299 (1994).

     K. Carlsson, A. Liljeborg,
     "Confocal fluorescence microscopy using spectral and lifetime
     information to simultaneously record four fluorophores with high
     channel separation", J. of Microscopy, Vol. 185, pp. 37-46 (1997).

     K. Carlsson, A. Liljeborg,
     "Simultaneous confocal lifetime imaging of multiple fluorophores
     using the Intensity-modulated Multiple-wavelength Scanning (IMS) technique,"
     J. Microsc. 191, 119-127 (1998).

     E. Bergman, K. Carlsson, A.  Liljeborg, E. Manders, T.  Hökfelt and
     B. Ulfhake "Neuropeptides, nitric oxide synthase and GAP-43 in
     B4-binding and RT97 immunoreactive primary sensory neurons: normal
     distribution pattern and changes after peripheral nerve transection
     and aging," Brain Res 832, 63-83 (1999).

You can also take a look at the following links:

    http://www.biox.kth.se/research/confocal/confocal.htm

    http://www.biox.kth.se/staff/anders/quadra.htm

    http://www.biox.kth.se/staff/anders/esbj.htm

The last link is showing results used in the last reference.

Best wishes

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	Assoc. Prof. Anders Liljeborg
	Biomedical Physics and X-ray Physics
	Teknikringen 14, 4 tr.
	Royal Inst. of Technology
	SE - 100 44 Stockholm, Sweden
	Phone +46 8 790 7219
	Fax.  +46 8 205 609
	E-mail: [log in to unmask]
	http://www.biox.kth.se
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