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What cells do you use?

We have used DAPI to label the nucleus in living cells in a number of
different human tumour cell lines.
Although I am sure a DAPI stained nucleus is not very physiological the
cells seem to be quite happy 12 hours later and endocytotic processes seem
to be still active.


Andreas


Andreas G. Schatzlein
Department of Medical Oncology
Cancer Research UK Beatson Laboratories
Garscube Estate
Bearsden
Glasgow
G61 1BD
Tel 0141 330 4354
Fax 0141 330 4127
E-Mail [log in to unmask]



-----Original Message-----
From: Confocal Microscopy List
[mailto:[log in to unmask]]On Behalf Of Oliver Lung
Sent: 21 March 2002 01:34
To: [log in to unmask]
Subject: is DAPI membrane permeable?


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Dear All,
        I am trying find a dye we have in the lab that would stain
dead (dying) cells. It appears DAPI would not stain my healthy cells
(within about 30') suggesting it is not permeable and it is just what
I need. However, I have read in published articles that DAPI is
membrane permeable and therefore should not work for my purpose. Any
suggestions or comments on this is greatly appreciated.

sincerely,

Oliver
--
Oliver Lung
Postdoctoral Associate
Boyce Thompson Institute
Cornell University
Ithaca, NY 14853
tel: 607-254-1369