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You might want to consider the DNA-specific stain DRAQ5
(available from Biostatus Ltd., UK).  It would be excitable at 638
nm (optimal is 647nm but it worked for us at 633nm) and emits
from 670nm to the low infra-red.  It is cell permeant so it can be
used in both live and fixed cells, although I have no experience in
using it with whole tissues.  Still, it might be worth a try and could
save you the RNAsing.

Good luck.

>We need to stain whole tissue for DNA and need to do this with a
>far red
>dye. Do people have experience using toto3, Syto 59 or 60 with o
>whole
>tissue with good penetration 2mm+ o  the 638nm laser line for
>excitation ?


******************************************************************

Marie Follo, Ph.D.
Core Facility
Dept. Internal Medicine I
Hugstetterstr. 55
Universitaetsklinik Freiburg
79106 Freiburg
Germany
Tel.: +49 761 270 6377
Fax: +49 761 270 6378
e-mail: [log in to unmask]
http://www.ukl.uni-freiburg.de/core-facility