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CONFOCAL MICROSCOPY

4-Day Intensive Hands-On Course

Website:
www.york.ac.uk/biology/technology-facility/imaging-cytometry/ic-courses/confocal-course/

Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing

8-11 September 2015
Technology Facility, Dept. Biology
University of York, UK

Instructors will include

Peter O'Toole, University of York
Jo Marrison, University of York
Chris Power, Carl Zeiss
Andrew Hubbard, Andor
and special guest lecture and practical session with Daniel Zicha (CRUK).

Tutorials and practical sessions will cover both basic and advanced
confocal microscopy. The course will enable participants to realise the
potential behind many developing confocal techniques and realise the
simplicity of applying the techniques to a broad range of sample types and
fluorescent labels. Both novice and more experienced users welcome.

Preliminary Programme
DAY AIMS
Day 1: Basics, Familiarisation, x,y,z and multicolour imaging.
Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on
laser scanning confocal.
Day 3: Advanced Techniques covering spectral unmixing and FRET with
fluorescent proteins.
Day 4: Further advances. More samples to explore confocal limits, and time
for participants own samples.

Thursday evening will include a special guest speaker and course meal.

The course will utilise many different sample types ranging from cultured
monolayers to plant cells. A diverse range of fluorescent labels will also
be used, which will include various fluorescent proteins (CFP, GFP and YFP)
and classic antibody labels. Over the four days, at least four confocal
microscopes (Zeiss LSM 510/710/780/880 on both an invert and upright
microscopes, Andor Revolution XD) will be utilised.

Accommodation (en-suite), breakfast and lunches will be provided for the
length of the course.  Evening meals will be provided on both Tuesday and
Thursday evenings.

Places are limited to 12 participants to permit full hands-on practice, so
please book early to avoid disappointment.
For further information, please visit the course website at:
www.york.ac.uk/biology/technology-facility/imaging-cytometry/ic-courses/confocal-course/

or contact Rachel Cullivan 01904 328821, [log in to unmask]

-- 

<http://www.linkedin.com/pub/peter-o-toole/1b/b79/444>
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : +44 (0)1904 328722
Fax : +44 (0)1904 328804
email : [log in to unmask]
www.york.ac.uk/biology/tf

Times Higher Education University of the Year 2010

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