At 08:59 AM 1/29/97 -0500, you wrote:
>Hi
>
>I was following the a discussion in the Confocal archives around 3/95
regarding deconvolution of images.  It was said at one point that to
accurately deconvolve an image, the complete system must be known.  Does
anyone know what exactly must be "known", how one goes about getting that
information, and is there software that can take that information to deblur
or deconvolve an image?
>
Hello,
There are a number of variations for deconvolution algorithms but they all
basically need information about the objective (N.A., working distance) and
the microscope setup,( refractive index of the medium wavelength of light
used etc.). All  of these fators affect a function called the point spread
function which describes how the image obtained from  a single point source
will appear.  There are various ways of calculating a PSF either
theoretically or empirically using fluorescent beads. For more details see
some of the papers by Agard (e.g. Meth. Cell Biol. (30), 353-377)
or Ann. Rev. Biophys. Bioeng. (13), 191-219).
I also have a paper that describes some of these methods and software to
calculate the PSF (Meth. Cell Biol. (40), 221-240).
Hope this helps
Dr. John Cork,
Calcium Imaging Facility
Department of Anatomy, LSUMC,
1901 Perdido St., New Orleans
LA 70112

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