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Date: | Fri, 17 Mar 2000 17:10:30 GMT |
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As Jurek says, the 457 nm line can be used to excite DAPI (See more
about this at the end). This line is now available from a four line
Argon laser offered as one of several laser options on our
Radiance2000 systems.
This laser has a total power of approximately 40 mW with power
distribution between the individual lines as follows:
457 nm 2.5 mW
476 nm 2.0 mW
488 nm 20 mW
514 nm 10 mW
The laser's expected lifetime is approximately 3,000 hrs.
The primary application for the 457 nm line is for imaging CFP
together with other fluorescent proteins such as YFP (using the 514 nm
line).
To our knowledge it is only possible to image DAPI in fixed
preparations with the 457 nm line. We believe that the excitation
spectrum of DAPI is shifted upon fixation.
Jurek, have you tried imaging DAPI at 457 nm in unfixed prep's?
Best regards
Duncan McMillan
Group Product Manager
Bio-Rad Microscopy Division
Hemel Hempstead
UK
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