Hello,

We use Feulgen stain to discriminate between chick cells and quail cells
based on a large staining appearance of the quail nucleoli. This is a
well established method in embryology for cellular fate mapping. Feulgen
stain is brightly fluorescent in the green HeNe wavelength and is
difficult to bleach. We even clear our tissue specimens to allow deep
z-scanning by confocal. Maybe it can also work in your case.

>Subject:     representing the nucleoli
>Sent:        05/18/1920 6:33 AM
>Received:    05/23/2000 5:42 PM
>From:        Hesam Dehghani, [log in to unmask]
>Reply-To:    Confocal Microscopy List, [log in to unmask]
>To:          [log in to unmask]
>
>Hi everybody,
>
>I am looking for a good visual way to show the difference in nucleolar
>size of cells in two experimental groups.
>Is there any way to reconstruct the nucleoli out of the cells ? These
>are confocal images with 100 stacks and
>nucleoli show the background gray level, however, the nucleoplasm and
>cytoplasm are positively stained with the
>antibody. Any suggestion would be greatly appreciated.
>
>
>Hesam Dehghani
>Department of Biomedical sciences
>University of Guelph
>Guelph, Ontario, Canada


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