Aaron,

Use the side output of your inverted scope, three coupled Newport
micrometer stages and a side window Hamamatsu PMT. You can get the
latter with built in power supplies that you run off of a variable 5
volt supply. Ted Pella and others sell pin holes of different
diameters. It will probably cost you about $3 k.


>Hi:
>
>I have a simple application: I am planning to use the
>confocal principle to increase S/N for my single-point
>fluorescence detection setup.  I do NOT need to scan
>or assemble an image; I am simply detecting from a
>single point.
>
>My question is one of logistics: do any of you know of
>a product that can be mounted on a Zeiss Axiovert 135
>inverted microscope that would allow convenient
>implementation of this scheme?  I imagine a simple
>product, consisting of
>
>(1) a lens
>(2) a pinhole mounted on a 3-axis manipulator
>(3) a PMT housing
>
>I hope that this would be a relatively inexpensive
>product because I do not need mirrors for scanning or
>image-assembling/processing software.
>
>Thanks for your help,
>Aaron
>
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--

Mario M. Moronne, Ph.D.
NanoMed Technologies, Inc.
ph (510) 528-2400
Berkeley, CA
94706