CONFOCALMICROSCOPY Archives

May 2000

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Subject:
From:
"m.cannell" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 25 May 2000 09:29:50 +1200
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Not filling the rear aperture produces a larger spot size. Simply, you
are effectively reducing the NA of the lens.

Mark Cannell

David Knecht wrote:

> Sorry for the dual posting.  I forgot to change the subject.
>
> Dear users- What is the effect of not filling the back focal aperture
> of an objective in a point scanning confocal system.  I am assuming
> the same laser power going in, but the beam not expanded as far.  It
> seems to me that it is a different issue than in a normal imaging
> system. Would you get a smaller spot of illumination?  Thanks- Dave
> --
>
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> "Home of the 2000 NCAA Women's Basketball Champions"
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> University of Connecticut
> 75 N. Eagleville Rd.   U-125
> Storrs, CT 06269
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