Hi Larry:
Good to hear from you, how's life in San FRancisco?
Many thanks for the ref.. I'm putting up a paper (with deadline tomorrow) in which I think we are the first ones to look at meiosis INSIDE THE MEIOTIC ORGAN (in this case plant anthers). So far it's only DAPI (which works wonderfully under 2P), but I think we proved the point that 2P has the potential to go that deep.

And how's your exploration of the no-linear photonic world? I've done some GFP (so far only wt, in roots, pollen tubes and yeast) and although we could surely image it, the fading rate is worst than with 1P, therefore so far I'm a bit disapointed. Also I tried chameleon for calcim , and again could not come with anything. How are you doing into that direction?

Thanks for your ref. You may remember that I e.mailed you when it came out, and you were kind enough to send me a reprint. Actually I had student doing a journal club with it.

Cheers
Jose

*********** REPLY SEPARATOR  ***********

On 24-05-2000 at 11:35 Larry wrote:

>Hi Jose! Here is the yeast reference:
>
>Microtubule Dynamics in Fission Yeast
>                Da-Qiao Ding and Yasushi Hiraoka
>  Kansai Advanced Research Center, Communications Research Laboratory,
>Kobe, Japan
>       www-karc.crl.go.jp/bio/CellMagic/movie
>
>REFERENCES & ACKNOWLEDGEMENTS
>
>Chikashige et al., 1994, Science 264, 270-273
>
>Ding et al., 1998, J. Cell Sci. 111, 701-712.
>
>Our lab had a recent publication that might be of interest:
>
>"Modes of Protein Movement that Lead to the Asymmetrid Localization of
>Partner of Numb during Drosophila Neuroblast Division" Bingwei Lu, Larry
>Ackerman, Lily Jan, Yuh Nung Jan, Molecular Cell, Vol 4, 883-891, December
>1999.
>
>Larry D. Ackerman
>Lily & Yuh Nung Jan Laboratories
>Howard Hughes Medical Institute
>UCSF, Box 0725, Rm U226
>533 Parnassus Ave.
>San Francisco, CA 94143
>
>(415) 476-8751  FAX (415) 476-5774
>[log in to unmask]





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Jose' A. Feijo', Prof.
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Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL

tel. +351.21.440.79.41, fax +351.21.440.79.70

and

Dep. Biologia Vegetal, Fac.Ciencias, Universidade Lisboa,
Campo Grande, Ed.C2, PT-1749-016 Lisboa, PORTUGAL

tel. +351.21.750.00.69, fax  +351.21.750.00.48
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e.mail: [log in to unmask]
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