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June 2000

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Subject:
Na sensitivity of Ca indicators ?
From:
Milton Charlton <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 7 Jun 2000 13:56:02 -0400
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Does anyone know of measurements of the Kd for Na+ of any of the Ca2+
indicators?  In R.Y. Tsien's paper in Biochemistry, 1980, (19, 2396-2404)
describing BAPTA type chelators, fig 3 shows that 430mM Na has a small
effect on the absorption spectrum of BAPTA compared to 10uM Ca2+ (fig 2).
Has anyone actually measured Kd for Na+ of Ca2+ indicators?
Thanks

Milt Charlton

Milton P. Charlton
Professor
Physiology Department
MRC Group
University of Toronto
1 King's College Circle
Toronto, ON
M5S1A8
Canada

tel: 416-978-6355
fax: 416-978-4940
http://www.utoronto.ca/physio/faculty/charlton.htm

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