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June 2000

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Subject:
Chromatin counter stain
From:
Steve Taylor <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 5 Jun 2000 15:32:23 +0100
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I'm running a MRC1024 (without UV laser) with excitation lines 457, 488,
514, 543 and 638. Regular fluorochromes used are FITC, Rhodamine, Cy5 as
triple labels for chromosomes.
Can anybody suggest a counter stain for DNA/chromatin so that the entire
chromosomes can be imaged when using triple labeling?.

Many thanks

Steve Taylor
---------------------------------

Dr. S. Taylor
CLSM Unit
Institute of Biological Sciences
Cledwyn Building
University of Wales
Aberystwyth
Ceredigion
SY23 3DD
UK

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01970 621567
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