Hello Leica users,

for my experiments I need to know the size of the pinhole of the Leica
TCS SP backprojected onto the sample. The software shows me a digital
pinhole size of 90 and claims this to be the size of one Airy disk
(Objective: 100x/1.3). However, I doubt this, as the Airy disk reading
doesn't change with the chosen excitation/emission wavelength. Maybe
Leica calculates the Airy disk reading for a fixed reference wavelength?
Can anybody help me?

Thank you in advance,

Joachim


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  Joachim Walter, Dipl. Phys.
  Institut für Anthropologie und Humangenetik der LMU München
  AG Cremer
  Richard-Wagner-Straße 10/I
  D-80333 München               Tel. +49 - 89-2180-6713
  Germany                       Fax               -6719

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