Hi All,

   We're going to be fixing and staining Xenopus cells to examine their
yolk platelets, and I was hoping someone has had good experience with lipid
fixation and suitable fluorescent lipophilic probes for this system.
Specifically, we don't want any of our yolk platelets to float away during
fixation and membrane permeabilization. I'm also curious as to which Di"X"
analogs and/or Bodipy's have worked best for vitellogenin and phosvitin
containing yolk. Thanks in advance.

Mike



Michael B. Ferrari

Department of Biological Sciences
University of Arkansas
Fayetteville, Arkansas 72701
Ph: 501-575-6372
Fax: 501-575-5349
email: [log in to unmask]
http://biology.uark.edu/ferrari