Susan,

I tend to agree with everyone you have already heard from. For ER and
Golgi staining I suggest looking at the Molecular Probe page:

http://www.probes.com/handbook/sections/1204.html

BTW, a side view projection or slice would be nice to see, also.

Mario
>Hello everyone,
>
>When transfecting a GFP fusion protein into NIH 3T3 cells (mouse
>fibroblasts), we are seeing distribution into large globular stuctures in
>the cell cytoplasm.  We have tried staining the lysosomes, but there is no
>colocalization.  Does anyone have any suggestions as to what these could be
>and of appropriate stains for colocalization?
>
>  <<Vadim SelK localization.doc>>
>
>Thanks,
>
>Susan Garfield
>Confocal Facility Manager
>Laboratory of Experimental Carcinogenesis
>Division of Basic Sciences
>National Cancer Institute, NIH
>Building 37, Room 3C28
>37 Convent Drive MSC4255
>Bethesda, MD  20892-4255
>+1 (301)496-5688 x227 (voice); +1 (301)496-0734 (fax)
--
_____________________________________________________________________
Mario M. Moronne, Ph.D.
NanoMed Technologies
ph (510) 528-2400
FAX (510) 528-8076
Berkeley, CA
94706