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January 2001

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Subject:
immunofluorescence in Arabidopsis
From:
Susanna Castel <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 15 Jan 2001 21:12:14 +0100
Content-Type:
multipart/mixed
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text/plain (382 bytes) , susana.vcf (339 bytes) 
Dear confocalists,

I'm looking for a good protocol for immunofluorescence techniques in
plant cells. Our immediate interest is to stain with antibodies some
structures as Peroxisomes, Golgi, ER, in Arabidopsis sheets that are
expressing some GFP-proteins. We would like to make a "whole mount"
method, but I'm not sure about fixation and permeabilization of the cell
walls.

Thank you


Susanna

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