CONFOCALMICROSCOPY Archives

January 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: C. elegans fixation, fluorescent proteins
From:
Rosemary White <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 19 Jan 2001 12:45:33 +1000
Content-Type:
text/plain
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Dear Guenter,

We have fixed plant tissue in 4% paraformaldehyde in 25 mM phosphate
buffer, up to overnight, and GFP looks fine.  You might get interfering
green fluorescence after glut fixation.

cheers,
Rosemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone  61-2-6246 5475
fax       61-2-6246 5000
email   [log in to unmask]

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