CONFOCALMICROSCOPY Archives

February 2001

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Subject:
colocalization
From:
Kees Jalink <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 28 Feb 2001 15:24:20 +0100
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Dear all,

we study the colocalization between simultaneously acquired images of 2
different fluorophores, one red, one green. These dyes are more or less
all over the cell, in a dark and bright spotted pattern. Rather than
just taking 'representative' pictures that show what we hope to show,
we'd like to express things a bit more quantitatively / statistically
justified. What methods do people use to quantify colocalization?

any comments wellcome.
Thanks, Kees

--

Kees Jalink Ph.D.
The Netherlands Cancer Institute, dept. of Cell Biology H1
Plesmanlaan 121, 1066CX Amsterdam, the Netherlands
020-5121933 (tel office)/ ...1947 (tel lab) / ...1944 (fax) /
[log in to unmask] (email)
at home: Paulus Potterlaan 5, 2102CC Heemstede
0235-476047 / [log in to unmask]

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