CONFOCALMICROSCOPY Archives

March 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
temperature of facs preps
From:
Heather Medbury <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 7 Mar 2001 14:34:09 +1100
Content-Type:
text/plain
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text/plain (24 lines) 
Hi FLOWers,

About 5 years ago when I was analysing lymphocytes, we used to have the cell preparations at 4°C for labelling with the antibody. 
The work I do now on whole blood, the cells are labelled at room temperature.

Why do people label at 4°C when it works perfectly well at room temp?

Heather

Heather Medbury (PhD)
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
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     All experiments are preliminary

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  "In his heart a man plans his course
    but the Lord determines his steps"

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