Hi, Dave,
The major issue with the XBO is the intensity in the region of the spectrum
needed for exciting your particular dyes.
Also, since both HBO and XBO are arc sources, as far as I know, the line
current is going to have an effect on either.
As for Ludl having a less expensive version... thanks for the
update. Needless to say, we can't keep up with all the changes in pricing,
etc., and appreciate help from knowledgeable end-users such as yourself.
Good to hear from you.
Best regards,
Barbara Foster
At 03:22 PM 3/5/01 -0500, David Knecht wrote:
>>Dear Barbara- I have been looking into this and Ludl has a much
>>cheaper stabilized source. Any negative thoughts? Also, is there
>>any real downside to buying a stabilizer as opposed to going with a
>>Xenon bulb? My reading of the discussion is that you don't need
>>anything fancy with Xenon in terms of power supply. Dave
>
>
>
>>Steve,
>>
>>OptiQuip has new stabilized Hg light sources. Many years ago I was the
>>East Coast microspectrophotometry specialist for Zeiss and used stabilized
>>HBO's but they have been very expensive. OptiQuip has extensive experience
>>in this area and makes lamp housings to fit most microscopes. If
>>interested, contact: Mel Decker - [log in to unmask]
>>
>>Caveat: no vested interest.
>>
>>Best regards,
>>Barbara Foster
>>Microscopy/Microscopy Education
>>125 Paridon Street, Suite 102
>>Springfield, MA 01118
>>PH: 413-746-6931 FX: 413-746-9311 Web: www.MME-Microscopy.com/education
>>
>>"Why didn't they teach us that sooner?" ... probably because no one
>>thought to call MME about customized, on-site courses. Offered in all
>>areas of microscopy, sample prep,and image analysis, they make an immediate
>>impact on your productivity.
>>@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
>>
>>
>>
>>
>>At 09:41 AM 2/28/01 +1100, Stephen Cody wrote:
>>> >From my observations of using both types of light source, the Xenon seems
>>>far more stable with time. You don't notice the fluctuations that you get
>>>with Hg bulbs. This is important for quantification, such as dual excitation
>>>ratio methods (eg. Fura-2).
>>>
>>>Stephen H. Cody,
>>>Colon Molecular and Cell Biology Laboratory,
>>>Ludwig Institute for Cancer Research,
>>>Post Office Royal Melbourne Hospital,
>>>Parkville, Victoria 3050, Australia.
>>>
>>>Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
>>>email: [log in to unmask]
>>>www.ludwig.edu.au/confocal
>>>
>>>
>>>> ----------
>>>> From: Tom Phillips[SMTP:[log in to unmask]]
>>>> Reply To: Confocal Microscopy List
>>>> Sent: Wednesday, 28 February 2001 2:08
>>>> To: [log in to unmask]
>>>> Subject: Re: Xenon lamps
>>>>
>>>> All true but I believe one disadvantage is the peak illumination is
>>>> less for some fluorochromes such as DAPI. We see a difference in our
>>> > two systems that have Hg or Xe.
>>> >
>>> >
>
>--
>
>************************************************************
>Dr. David Knecht
>Department of Molecular and Cell Biology
>University of Connecticut
>75 N. Eagleville Rd. U-125
>Storrs, CT 06269-3125
>[log in to unmask]
>860-486-2200 860-486-4331 (fax)
>home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
>************************************************************
|
