CONFOCALMICROSCOPY Archives

March 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
David Biggs <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 23 Mar 2001 14:17:27 -0500
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Hi,

I would like to use the combined wisdom of the confocal mailing list to find
some quantitative information about the physiology of yeast cells, (and
being an electrical engineer, this is a little outside my scope of
knowledge!)

Specifically I would like to know about the refractive indicies of the
various cell components in budding yeast cells.  There appear to be three
main components:
        - cytoplasm
        - nucleus
        - centrioles
In DIC the nucleus appears to have a lower refractive index w.r.t. the
surrounding cytoplasm, and the small round centrioles have a much higher RI.

If anyone has information or a reference, I would greatly appreciate
knowing.  Please email the list or myself directly ([log in to unmask]).

Thanks in advance,
David
---
David Biggs, PhD.
R&D Scientist
AutoQuant Imaging, Inc.
[log in to unmask]

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