At 11:15 08/05/01 +0200, you wrote:
>Dear Matthias, dear all,
>
>there is definitely no limitation to 10 steps in Lambda Scan (spectral
scan) -
>you can do as many steps as you like to do!
>Please find attached a protocol how to do Lambda Scan with TCS NT software
with
>Leica TCS SP.
>As Holly pointed out, the use of Lambda Scan in new software LCS is much more
>user-friendly as it was in old software version.
>Ulrike
>
>(See attached file: LambdaScan.pdf)
>
>_________________
>Ulrike Tauer, Ph.D.
>Application Specialist
>
>Leica Microsystems Heidelberg GmbH
>Am Friedensplatz 3
>68165 Mannheim
>Phone: +49 (0)621 7028 2704
>Fax: +49 (0)621 7028 2780
>mailto:[log in to unmask]
>
>
>
>
>
>
>
>
>
>Stamatis Pagakis <[log in to unmask]> on 29.04.2001 19:15:06
>
>Please respond to Confocal Microscopy List
<[log in to unmask]>
>
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> To: [log in to unmask]
>
> cc: (bcc: Confocal News/DEMAN/LMSCentral/Leica)
>
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>
> Subject: Re: Emission spectra with Leica TCS SP?
>
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>Hi
>
>the way to do it is to set-up a number (I think 10 is the maximum the
>software accepts) acquisition methods, each having identical parameters
>except the emission bandwidth. Do then sequential acquisition with all
>methods. You will collect a sequence of 10 images, each representing the
>signal at the selected bandwidth. If you plot the integral within an ROI
>for each of your images, you end up with a spectral plot of your
>fluorophore. Hope it helps. Sorry for the brief description, but if you
>need more info, please get back to me
>
>regards
>
>*-----------------*------------------*----------------------*---------------*
>
>>Dr Stamatis Pagakis [log in to unmask]
<
>>Confocal and Image Analysis Laboratory Tel: +44 (0)20 8913
8675 <
>>Membrane Biology Mobile: 07867 500029
>>National Institute for Medical Research Switchboard: 020 89593666
x2621 <
>>The Ridgeway Message: x2219, x2622
<
>>Mill Hill, London NW7 1AA FAX: +44 (0)20 8906
4477 <
>
>
>
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>On Fri, 27 Apr 2001, Holly Aaron wrote:
>
>> Hi, Matthias -
>>
>> It is possible. I think with the TCS software that you'd have to set up
>> either a time series with different wavelengths, i.e., manually selecting
>> different wavelength ranges, or some sort of sequential scanning, also
>> setting your own. <i have never done it on the old software and my
>> memory of that software is fading, but feel free to contact me offline and
>> i can go into more detail on how to do that>
>> The newer (much nicer) software from Leica - LCS - has a built-in function
>> just for that - xy(z)-lamda (xyz-wavelength) - and it is quite elegant.
>> You can specify the range, i.e. 500 to 600nm, the filter-width (10nm or
>> whatever), and the number of steps to take (i.e. 10 to cover all
>> wavelengths in that range). Then it's easy to plot out a graph from the
>> data. I would highly recommend upgrading to the new software.
>>
>> Good luck,
>> -Holly
>>
>>
>> Matthias Heinemann wrote:
>>
>> > Hello!
>> >
>> > We wanted to determine the emission spectra of a dye with our
>> > CLSM (Leica TCS SP with the software Leica TCS NT Version
>> > 1.6.582).
>> >
>> > I am pretty sure that the our Leica representative had once
>> > demonstrated a method (something like a time series with
>> > changing detection wavelenghts ...). But, unfortunately I cannot
>> > remember who is was done.
>> >
>> > I would be glad if someone could help me!
>> > Thanks a lot in advance.
>> >
>> > Best Regards,
>> > Matthias Heinemann
>> >
>> > --
>> > -----------------------------------------------------------------------
>> > Dipl.-Ing. Matthias Heinemann Aachen University of Technology (RWTH)
>> > Department of Biochemical Engineering
>> > Tel: +49 241 80-7072 Worringerweg 1
>> > Fax: +49 241 8888-265 52074 Aachen, Germany
>> >
>> > [log in to unmask] http://www.biovt.rwth-aachen.de
>> > -----------------------------------------------------------------------
>>
>> --
>> Holly L. Aaron
>> Berkeley Imaging Center
>> http://imaging.berkeley.edu/
>>
>
>
>Attachment Converted: "C:\EUDORA\Attach\LambdaScan.pdf"
>
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Arnold Richard Pizzey
Department of Haematology
Royal Free and University College London Medical School
98 Chenies Mews
London WC1E 6HX
U.K
voice: +44 020-7679-6234
Fax: +44 020-7679-6222
email: [log in to unmask]
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