CONFOCALMICROSCOPY Archives

July 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
"Vergara, Leoncio A." <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 26 Jul 2001 10:35:28 -0500
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Has anybody experienced the same problems with the new Axiovert 200M?

-----Original Message-----
From: F Javier Diez Guerra [mailto:[log in to unmask]]
Sent: Thursday, July 26, 2001 10:17 AM
To: [log in to unmask]
Subject: Re: unstable focus on Axiovert 100


Dear Listmembers,

We are currently doing time-lapse series during 2-4h periods using Axiovert
100 and Axiovert 135 estatives and have the same problems. The focus drift
is of such magnitude that it forces us to be constantly (every 4-5 min)
adjusting the micrometric control manually. One of the micros is set on a
high-inertia table and we are using closed perfusion chambers with its own
temp control.

It would be of great help to know how other colleagues have resolved this
issue.

Best regards,


F Javier Diez-Guerra
Servicio de Microscopia Confocal
Centro de Biologia Molecular Severo Ochoa
Facultad de Ciencias, UAM
28049 Madrid, Spain

Tel     34-913978051
Fax     34-913978087

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