Why is he using a mounting medium which dries?  It seems as though it
is introducing an additional and unnecessary variable.  How is one to
know whether it dries completely to the same extent everywhere?  If
not, one will expect to have a variation in the refractive index from
one place to another.
Carol Heckman


>Confocal listservers,
>I have a user who is using a 10x objective to image nerve tracks in brain
>tissue. He is using an aqueous mounting media which completely dries. On some
>preps he gets what looks like moire lines across his image. I have seen this
>before and thought it maybe caused by drying artifacts between coverslip and
>sections (which are picked up on the slide not the coverslip). Does anyone
>have any suggestions which precludes the formation of these moire? lines and
>an explanation of their formation. Also, they seem to be wavelenght dependent:
>more pronounced with the 543 line than the 488 line. Is there a relation with
>excitation or emission?
>
>Hank Adams
>Integrated Microscopy Core
>Molecular and Cellular Biology
>Baylor College of Medicine
>Houston, Tx
>
>Hank Adams
>Manager
>Integrated Microscopy Core
>Molecular and Cellular Biology
>Baylor College of Medicine
>Houston, Tx 77030