Hi Allen,
if you need a slider for testing (especially DAPI) - the below might be of
interest for you.
mfg / regards
Anneliese Schmaus
Product Manager
klughammer gmbh
Strassbach 9
85229 Markt Indersdorf
Germany
Tel. +49 08136 6011
Fax +49 08136 7098
www.klughammer.de
DT> Barbara:
DT> We supply a three slide set similar to what you describe below for
DT> illumination calibration.
DT> One slide for the lower DAPI like probes, one for the FITC,Rhod, wavelength
DT> probes, and another for the higher wavelength probes including Cy5.
DT> If you are interested, you can contact: [log in to unmask]
DT> Set of Fluorescent Calibration Slides 52-503256-003 $60.00
DT> Regards,
DT> Tom
DT> Tom Donnelly Applied Precision, Inc.
DT> Biotechnology Group 1040 12th Ave. N.W.
DT> (425)557-1055 fax Issaquah, WA 98027-8929
DT> [log in to unmask] http://www.api.com/products/bio/deltavision.html
DT> -----Original Message-----
DT> From: Barbara Foster [mailto:[log in to unmask]]
DT> Sent: Tuesday, October 02, 2001 12:57 PM
DT> To: [log in to unmask]
DT> Subject: Re: Confocal QA or Pretty Pictures
DT> Hey, Ray,
DT> MME is in the process of putting together just such a slide set:
DT> 4 slides which have spectra similar to FITC, Rhodamine/Texas Red, Acridine
DT> orange, and DAPI.
DT> Is that the sort of thing you need?
DT> Best regards,
DT> Barbara Foster
DT> Microscopy/Microscopy Education
DT> 125 Paridon Street, Suite 102
DT> Springfield, MA 01118
DT> PH: 413-746-6931 FX: 413-746-9311 Web: www.MME-Microscopy.com/education
DT> "Why didn't they teach us that sooner?" ... probably because no one
DT> thought to call MME about customized, on-site courses. Offered in all
DT> areas of microscopy, sample prep,and image analysis, they make an immediate
DT> impact on your productivity.
DT> @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
DT> At 10:24 AM 10/2/01 -0500, ray hester wrote:
>>Ten years or so ago when we purchased our first CLSM, an ACAS 570, Meridian
>>Instruments, the manufacturer, supplied us with a plastic slide impregnated
>>with a 'fluorescent' compound that could be used to monitor your system's
>>performance on a day-to-day basis. You set the laser power, PMT gain,
DT> etc.,
>>to the same settings each day, focused the same distance into the slide,
DT> and
>>clicked the space bar and it provided a graph of fluorescence vs AOM
DT> setting
>>(from 0 to 100) which could be tracked.
>>
>>Even something as primitive as a fluorescent slide might be a help with our
>>new confocal, although certainly we/they are capable of a more
DT> sophisticated
>>system today.
>>
>>Ray Hester
>>Univ. of South Alabama
>>[log in to unmask]
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