Try the camera first. You can't really make a decision based only on the
specs.  It may well only catch the tail of Cy5...but that may be enough
for you.

Even with the SPOT RT I could see _some_ Cy5.

Jean Brennan wrote:
>
> Hi Owen,
>
> The filter in the Zeiss color camera is what worries me too.  I think it's going to be close, but after talking to the rep I think it will work.  I'm going to choose Fluorophores that are closer to 630 than 690.  We got the camera for BF and some fluorescence, but it looks like we'll be needing to get a monochrome camera soon.
> Thanks for your help,
> Jeanne   :)
>
> Jean Brennan
> Imaging and Microscopy Core Facilitator
> Trudeau Institute, Inc.
> PO Box 59
> Saranac Lake, NY  12983
> Phone: (518) 891-3080
> FAX: (518) 891-5126
> [log in to unmask]
> www.trudeauinstitute.org
>
> >>> [log in to unmask] 11/30/01 12:18PM >>>
> Dear All,
>
>         We use CY5 quite often with a 100w HG lamp for excitation.  Using
> the CY5 filter cube, we can collect images on a Princeton Micromax with no
> problems.  My only question would be with the Zeiss color camera.  Is there
> a way to remove any color filters in the beampath, or is is a masked ccd
> chip?  I always advise users to collect fluoro images as monchrome and
> pseudo color later.
>
> Cheers,
>
> Owen
>
> Owen M. Schwartz
> Head, Biological Imaging Facility
> NIAID, NIH
> Bldg 4  Room 424   MSC 0485
> Bethesda, MD  20892  USA
>
> Phone 301-402-5883
> Fax 301-402-0366
> email: [log in to unmask]

--

     ________________________________________________________
    / Michael J. Herron,  U of MN, Dept. of Pediatrics/BMT  /
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