Greetings,
I have a user who is immunolabeling bacterial inclusions with a fluorescein
conjugate.  We would like to find a counterstain which would allow us to
put the inclusions in context (currently we can get a transmitted light/DIC
image for overlay, but it is not confocal, and we would like to
re-construct a 3D image from an image stack).
         Does anyone have suggestions for a good general purpose
counterstain for GM- bacterial cell perimeters which emits in either the
blue or red parts of the spectrum? A stain which specifically labels the
cell wall or membrane would be preferred to a nucleic acid stain.  Thanks.
-Karl G.

_______________________________________________
Karl Garsha
Specialist in Light Microscopy
Beckman Institute for Advanced Science and Technology
405 North Mathews Avenue
Urbana, IL 61801
Room B650J
Tel: (217) 244-6292
Fax: (217) 244-6219
www.itg.uiuc.edu