With all the current discussions relating to choice of confocal
microscope, detection sensitivity and upgradability you might be
interested to know that Bio-Rad will be holding an Exhibition Showcase
at the American Society of Cell Biology meeting in Washington on
Monday 10th December, Room 13/14 at 4.00-7.00pm. In addition, at the
stand there will be a Radiance2100 with the new 405nm blue laser diode
for e.g. CFP and DAPI imaging and the SELs option for high-viability
live cell imaging and increased sensitivity. There will also be the
chance to see the new Clonis live cell isolation and laser processing
system.
Please get your tickets from the Bio-Rad stand (212-217).
Invited speakers at the Showcase will be:-
S. Reichelt and W.B. Amos . MRC LMB, Camb. UK.
The practical use of reduced confocal performance and increased signal
strength: comparison of a SELs-equipped point scanning confocal
microscope with the Yokogawa/Perkin Elmer.
Prof. Ammasi Periasamy: Univ. Virginia
A study of protein interactions using two-photon excitation FRET
microscopy
J.C. Bouwer and Prof. M.H. Ellisman. UCSD, San Diego, California.
High speed two photon excitation microscopy (TPEM) using the Bio-Rad
RTS2000 video rate scanning microscope.
David Morris. Bio-Rad Microscience Ltd. UK
A system for live cell isolation and laser processing of tissue in
vitro.
There will be refreshments served too. We look forward to seeing you
there.
Regards
Eric Pierce Ph.D.
Product manager
Bio-Rad Microscience
www.microscopy.bio-rad.com
______________________________ Reply Separator _________________________________
Subject: Zeiss META
Author: <[log in to unmask]> at Internet
Date: 12/6/01 11:57 PM
Stil on the subject of the Zeiss META, I was wondering if someone in the list ha
s
actually detailed hands-on info on the system. We're in the process of buying a
new confocal, and this is an expensive option (on top of Zeiss being the most ex
p
ensive per se). I understand the technicalities and the potential for multispect
r
al imaging, but would like also to hear practical details, like reliability, mer
g
ing with the software, detection comparisons (Zeiss claims it's the same, but go
i
ng through a grating filter and spliting the available photons between an array
o
f PMT's sounds hardly like a way of increasing sensitivity) and any other detail
s
that may make one decide whether or not it's worth the money.
I was kind of hesitating to bring up to the list another "I have to buy a new on
e
- HELP!" request, it's a sort of re-current issue that I believe most people get
s
bored with. Anyway since I found a METAexcuse, let me extend the quest for help
to anyone that has been recently passed through the hellish process of having to
make the usual BioRad-Leica-Zeiss decision. Please mind that I would value more
r
ealistic oppinions of realistic hands-on, paper-publishing, biology users than s
t
rictly technical oppinions, I've been getting a lot of the latest. Specially wel
l
comed are:
1. Direct side-by-side confrontation of Bio-Rad and Leica on the usual issues (s
e
nsitivity, resolution, separation, etc.)? (2100 vs. SP2)
2. If Bio-Rad 2100, what optics? I only hear about Nikon, did everyone gave up o
n
trying others on the Bio-Rad?
3. If Bio-Rad, how well is their software coping with Leica and Zeiss? Is it use
r
-friendly enough for a multi-user facility in which the regular users considers
a
noying and time-consuming anything further than pushing a button on a screen?
4. If Bio-Rad how is the engineering vs. the others? Bio-Rad has a tradition of
h
aving to pull 10 knobs and filters to get the thing going, while the others usua
l
ly integrate much better the optics, does that stands in the 2100?
5. If Leica, does anyone feels limited by the objective inferior working distanc
e
s, specially on live imaging?
6. If Leica, I've heard some negative comparisons with the 2 others (SP2, 510 an
d
2100) on the issue of sensitivity, does anyone stands agaisnt or confirms this?
7. If Zeiss, any other really strong argument besides the software?
8. 10 good reasons to pay a lot more money for a Zeiss or a Leica over a Bio-Rad
?
basal config we are comparing for all brands includes:
- 3 lasers (Red solid state in the BioRad)
- AOTF
- 3 PMT's (2+1 arrayPMT in the ZeissMETA)
thanks in advance for any help in any of these points. The matter has some urgen
c
y, decisions have to be taken quite quickly, so I would appreciate quick, even i
f
short answers.
Cheers
Jose
**********************************************************
Jose' A. Feijo', Prof.
----------------------------------------------------------
Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL
tel. +351.21.440.79.41, fax +351.21.440.79.70
and
Dep. Biologia Vegetal, Fac.Ciencias, Universidade Lisboa
PT-1749-016 Lisboa, PORTUGAL
tel. +351.21.750.00.69, fax +351.21.750.00.48
__________________________________________________________
e.mail: [log in to unmask]
URL: http://193.126.26.2/Groups/plant_development.html
**********************************************************
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