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Wow.  No obj heater?  Our deltavision/bioptechs combination won't work
without it (keep cells alive, stable focus, etc).  (it's on a zeiss if that
matters)...

my 2 cents,

Michael A. Mancini, Ph.D.
Department of Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX  77030
713 798 8952
713 798 8005 (fax)
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http://microscopy.bcm.tmc.edu

-----Original Message-----
From: Confocal Microscopy List
[mailto:[log in to unmask]]On Behalf Of Alison North
Sent: Tuesday, January 08, 2002 2:17 PM
To: [log in to unmask]
Subject: Re: Bioptechs Focht Chamber System 2


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Hi Dave,

I don't use any objective heater - it seems to be stable without one and
those things make me nervous!  The DeltaVision is mounted on an Olympus
IX-70 microscope.

Glad to hear that your FCS2 works well,

Best wishes,
Alison

At 02:59 PM 1/8/2002 -0500, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> >I haven't tested the Bioptechs FCS2 system thoroughly so I can't comment
on
> >that, but I can highly recommend the Warner Instruments RC-30 confocal
> >imaging chamber which I have been testing out for them over the past few
> >months.  Like all closed systems it is a bit tricky to assemble, but the
> >temperature equilibrates within minutes and it is then extremely
> >stable.  Using it in conjunction with my DeltaVision microscope I have
> >collected images over periods of 4 hours or more without needing to
> >re-focus once (and this is using a x60 or x100 lens).  We have also used
it
> >successfully for 24-48 hour periods with perfusion, though the perfusion
is
> >rather fiddly.
>
>Hi Alison,
>
>I was wondering if you use an objective heater? or if it's that stable
>without one - also which microscope do you use with the DeltaVision?
>
>We have an olympus microscope and the FCS2 which seems to work pretty well
>- but we haven't done that much time lapse at this point.
>
>thanks
>
>dave smith
>Harvard University
>Imaging Center