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Hi Susan,

I haven't tested the Bioptechs FCS2 system thoroughly so I can't comment on
that, but I can highly recommend the Warner Instruments RC-30 confocal
imaging chamber which I have been testing out for them over the past few
months.  Like all closed systems it is a bit tricky to assemble, but the
temperature equilibrates within minutes and it is then extremely
stable.  Using it in conjunction with my DeltaVision microscope I have
collected images over periods of 4 hours or more without needing to
re-focus once (and this is using a x60 or x100 lens).  We have also used it
successfully for 24-48 hour periods with perfusion, though the perfusion is
rather fiddly.

No commercial interest - but when you find something that works it's worth
passing on the good news.

Good luck finding a system you like!
Best wishes,
Alison

Alison North
Director of the Bio-Imaging Facility
The Rockefeller University
1230 York Avenue
New York
NY 10021
USA
tel: (212) 327 7488


At 04:38 PM 1/8/2002 +0000, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Has anyone used Bioptechs FCS2 system? How do you like it? Are there any
>other closed systems around?
>
>Susan Puhalla
>Microscopy Research Assistant
>MRC Clinical Sciences Centre
>Room 5013
>Hammersmith Hospital
>DuCane Road
>London W12 ONN
>UK
>Telephone: 020 8383 8232