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     Dear Carol,
     I worked on fetal lung tissue for a while.  A good reference is Jeanne
     Snyder at the University of Iowa. The imaging was with
     epifluorescence, but the staining may be applied for confocal
     Here are the references:

     Am J Respir Cell Mol Biol 2000 Apr;22(4):432-40
     Exp Lung Res 1995 Nov-Dec;21(6):917-39
     Pediatr Pathol Mol Med. 2001 Sep-Oct;20(5):349-66.

     Hope this Helps

     Kavita Khubchandani Aswani PhD
     Applications Specialist
     Bio-Rad Microscopy Division
     Bio-Rad House
     Maylands Ave, Hemel Hempstead
     HERS HP2 7TD, United Kingdom
     Tel +44 (0) 208 328 2118
     e-mail: [log in to unmask]


______________________________ Reply Separator _________________________________
Subject: lungtissue
Author:  Confocal Microscopy List <[log in to unmask]> at
Internet
Date:    1/11/02 7:10 AM


Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I have an investigator interested in looking at lung biopsies using the
confocal microscope.  Does anyone have methods for fixation and staining of
this tissue or references for this type of specimen?

   Thank you,
        Carol

Carol D. Stearns
Biology
175 Briar Ridge Rd
Ridgefield, CT 06877
Tel:  203 798-5198
Fax: 203 791-6196
E-mail: [log in to unmask]






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