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Karl,
        Perhaps there is some relevant help at the following site:


http://www.dupontteijinfilms.com/datasheets/mylar/productinfo/properties/h37
237-1.pdf

        It's not precisely the same material, but it appears to be the same
polymer.

Hope this helps.  If not, I have to believe that Corning would have the same
type of data for the product you are using.

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
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> ----------
> From:         Karl Garsha
> Reply To:     Confocal Microscopy List
> Sent:         Thursday, March 21, 2002 1:57 PM
> To:   [log in to unmask]
> Subject:      Near IR quenching: polyester membranes
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Does anybody have information pertaining to the absorbtion spectra of
> polyester tissue culture membranes?  Specifically, I'm looking at cell
> membranes labeled with FM 4-64 on cells cultured on Corning-Costar
> Transwell Clear membranes.  The emmission spectra of FM 4-64 appears to be
> attenuated above 650nm in this instance, so I am wondering if the
> polyester
> membranes are quenching fluorescence from FM 4-64. The emmission spectra
> of
> FM 4-64 is supposed to peak at about 750nm, I'm getting peak emmission at
> 650nm and then a drop to baseline. Thanks in advance.
> -Karl G.
>
> _______________________________________________
> Karl Garsha
> Light Microscopy Specialist
> Imaging Technology Group
> Beckman Institute for Advanced Science and Technology
> University of Illiniois at Urbana-Champaign
> 405 North Mathews Avenue
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> Tel: (217) 244-6292
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>
>