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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
We have our UV fiber coupled to our SP-2. Our (femtosecond) MP is directly
coupled. The fiber coupling cuts down laser power by about 70%, and we run
some risk of damaging our UV fiber through turning up the UV output at the
laser. Photobleaching takes a while with all our fiber coupled lasers.
-Karl
At 10:41 AM 3/7/2002 +0100, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Is the UV laser relally fibre coupled in the Leica systems?
>I thought that it is directly coupled? We have a SP, with a directly
>coupled 2P laser, I am not sure, but I thought the UV is also directly
>coupled, and the photobleaching argument would not count?
>Arthur
>
>At 19:45 06.03.2002 -0500, you wrote:
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Hi Tom,
>>We have a Leica SP2, it was installed in August, and we have had very few
>>problems with it. We have had a few problems with the z-stage, but Leica
>>usually gets a service rep out within a few days. The scan head is fiber
>>coupled to the lasers, which means a couple of things: it lets you switch it
>>between different stands (an upright and an inverted), but it also means that
>>laser intensity is drastically reduced. This isn't a problem for
>>imaging, but
>>it does take a little while to do photobleaching, and probably won't have
>>enough power to do uncaging experiments (we don't have a UV laser, so this is
>>pure speculation). On the plus side of things, the system is very user
>>friendly, and the software is almost point and click (it usually only
>>takes new
>>users a couple of hours to get good at using the scope). The prism-based
>>emission detection system is much easier to use than filter based
>>systems, and
>>allows you to fine tune the separation of emissions. In general I'm very
>>happy
>>with the system and would reccomend it. If you have any specific questions,
>>I'd be happy to answer them.
>>Cheers,
>>Russell
>>
>>
>>
>>--
>>Russell McConnell
>>Confocal Imaging Facility Technician
>>Tufts University School of Medicine
>>Dept. of Neuroscience
>>M&V Building room 137
>>phone: 617-636-3795
>>http://www.neurosci.tufts.edu/Imaging
>>
>>Quoting Tom Gore <[log in to unmask]>:
>>
>> > Search the CONFOCAL archive at
>> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> >
>> > We are looking for thoughts from list members about purchasing a
>> > modern
>> > LSMs, especially from Nikon and Leica. We would be very interested in
>> > comments from users who have recently bought one. User-friendliness,
>> > ease
>> > of operation and need for little maintenance will be major factors
>> > that
>> > will influence the final decision.
>> >
>> >
>> > _____________________________________
>> > Tom Gore, Advanced Imaging Laboratory
>> > Biology Department, University of Victoria
>> > Box 3020, Station CSC,
>> > Victoria, BC, V8W 3N5 Canada
>> > voice (250) 721-7134 fax (250) 721-7120
>> > web: http://web.uvic.ca/ail/
>> >
>
>_______________________________________________
>Karl Garsha
>Light Microscopy Specialist
>Imaging Technology Group
>Beckman Institute for Advanced Science and Technology
>University of Illiniois at Urbana-Champaign
>405 North Mathews Avenue
>Urbana, IL 61801
>Room B650J
>Tel: (217) 244-6292
>Fax: (217) 244-6219
>www.itg.uiuc.edu
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