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Well,  you might want to try the following: you can quench the
autofluorescence for a while, if you flash a couple of times with a flash
of a photocamera.
I've been trying it at our lab to quench muscle tissue (the myofibril has a
very high autofluorescence), I used a Metz-45C flash and flashed about 10
times.  This flash has a pretty high intensity and quenches over the total
lightspectrum.  I did notice that the autofluorescence returns after a
while (time-depending on the tissue etc.), but I had enough time to make
some nice scans with a much lower intensity of the autofluorescence.
I'd say, give it a try!  :-)  Good luck,

Sven Terclavers

____________________________________________________________________
Sven Terclavers
Research Assistent
Center for Molecular and Vascular Biology
Center for Transgene Technology and Gene Therapy
Campus Gasthuisberg O/N Level 9
Herestraat 49 - 3000 Leuven - Belgium
E-mail: [log in to unmask]
____________________________________________________________________

At 08:08 15/03/2002 -0600, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>All,
>
>Our tissue cryosections often have "too much" autofluorescence.  We
>suspect it is largely due to collagen. Has anyone come up with a way to
>stain the collagen and thereby quench it's autofluorescence?
>
>Thanks
>Mike
>--
>
>      ________________________________________________________
>     / Michael J. Herron,  U of MN, Dept. of Pediatrics/BMT  /
>    / [log in to unmask]                                      /
>   / 612-626-4321   Mpls MN 55455                          /
>  /_______________________________________________________/