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Hi all
i have a Zeiss axiovert 100 with a scanning stage which i use to detect
single molecules in an epi-illumination configuration.
now i want to do this on glass slides evaporated with ITO (conducting
glass) ~1mm thick, and i'm looking for the right objective for the job. i
need high NA to get a small excitation spot and efficient collection and at
the same time a rather large working distance.  the best one i found is the
zeiss Ld acroplan 63X na 0.75. is this really the best NA i can get?
has anyone worked on this kind of substrate before?, are there any specific
considerations? is there a source for ITO evaporated cover slips?

tips from experienced microscopists will be appreciated
best regards
yuval




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  Yuval Ebenstein
  Department of Physical Chemistry
  The Hebrew University Of Jerusalem
  Jerusalem 91904, Israel
  TEL.:972-2-6586814 or 6584519
  FAX: 972-2-5618033
  mailto:[log in to unmask]
http://chem.ch.huji.ac.il/~nano/

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