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Hi,

I implemented my own tracking software a couple of years ago, based on
the Kanade-Lucas-Tomasi Feature Tracker (KLT) and it performs very well.
Once you have a software library to create movies and afterwards extract
frames for video analysis it is not so hard to do. If the objects of
interest show considerable shape variation, software based on a mean
shift tracker could be a nice option.

Another important issue is to sample the moving objects at the
appropriate rate and spatial density, so your software can find the
spatial and temporal relation between the moving objects.

More information about the Kanade-Lucas-Tomasi Feature Tracker (KLT):
http://vision.stanford.edu/~birch/klt/

Best regards,

Dr. Peter Van Osta

Union Biometrica N.V./S.A.
European Scientific Operations (ESO)
Cipalstraat 3
B-2440 Geel
Belgium
tel.: +32 (0)14 570 619
fax.: +32 (0)14 570 621

http://www.unionbio.com/

P.S. I have no commercial relation or interest in the Metamorf software.

>
> ----------------------------------------------------------------------
>
> Date:    Mon, 3 Jun 2002 11:37:26 -0400
> From:    "Goodhouse, Joseph" <[log in to unmask]>
> Subject: Tracking software
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>         Looking for tracking software to measure rates and variations of
> rates of movement of particles and organelles and leading edges from time
> sequence confocal images of live cells.  Any other software other than
> Metamorph that will do this?  Would like feed back from Metamorph users as
> to ease of use, problems, limitations etc.
>
> Joe Goodhouse
> Confocal / EM Core Laboratory
> Department of Molecular Biology
> Princeton University
> 609-258-5432
>
> Visit us at  http://www.molbio.princeton.edu/facility/confocal/index.html
>