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Dear Daniel,

we use Alexa conjugates of wheat germ agglutinin or Concanavalin A for
membrane labeling. For live cells we have excellent results with FM 4-64
and FM 5-95. All from Molecular Probes.

Cheers, Jens

Daniel Scharlau wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear all,
>
> I am looking for a good way to label the cell membrane of cultured
> cells. I am working mainly on PFA-fixed cells, but sometimes with living
>
> cells. So a vital stain would be nice, but is not necessarily required.
> I have tried the DiO cell labeling kit from Molecular Probes, but I was
> not at all satisfied, because the staining was not uniform and I had
> many problems with bleaching.
>
> I would be very thankful for any suggestions no matter if it's direct
> stains or an antibody. I am also interested in staining the apical
> membrane of epithelial cells exclusively, so if you have experiences
> with that, please let me know.
>
> Thank you in advance,
> Daniel.

--
Dr. Jens Rietdorf
EMBL, Meyerhofstr.1,
D-69117 Heidelberg,
Cell Biology/Biophysics Program,
Advanced Light Microscopy Facility
Phone ++49(0)6221 387-467 FAX-306