Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Try the 3I software site.
www.intelligent-imaging.com
They have MAC and PC solutions for 3-D analysis.
Anolik, Jennifer wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Does anyone have information on analyzing the colocalization of two
>fluorescently labeled antigens? I have been trying to do this using Leica
>LCS software (version 585) with little success. This software can generate
>a 2D plot of the fluorescent intensities and statistically analyze the
>localization of pixels. However, I can not get anything remotely resembling
>a colocalization coefficient. I have seen this published using Ultraview
>LCI software.
>
>I appreciate any input.
>
>Jennifer Anolik, MD, PhD
>Rheumatology/Immunology Unit
>University of Rochester Medical Center
>
>