Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

This suonds interesting.  But I would imagine that a chemical
reaction in solution would be accompanied by such rapid diffusion
that ion indicators would be changing throughout the solution at
essentially the same time.

So why image it?  If the person needs femtosecond imaging, he/she
would do well to find an expert in that area.
Carol Heckman
(Bowling Green State University)


>earch the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Greetings,
>I have a user (advanced chemical systems) who is interested in imaging the
>progress of a chemical reaction in solution using ion indicators.  I've
>pointed out some of the various fluorescent ion indicator systems I am
>aware of, but I am not sure how well they will hold up under
>non-physiological conditions.
>         I've posted this query under the remote possiblility that someone
>out there has worked with ion indicators at ~pH 12.00.  Thanks in advance
>for any advice.
>-Karl G.
>
>_______________________________________________
>Karl Garsha
>Light Microscopy Specialist
>Imaging Technology Group
>Beckman Institute for Advanced Science and Technology
>University of Illinois at Urbana-Champaign
>405 North Mathews Avenue
>Urbana, IL 61801
>Room B650J
>Tel: (217) 244-6292
>Fax: (217) 244-6219
>www.itg.uiuc.edu