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Hi -
I would very much appreciate some sage advice on this: we have been
using Leica TCSNT software with our confocal and are now trying to switch to
LCS. It feels like the right thing to do, the software is great. BUT, we are
having trouble when it comes to saving images, then importing them into
Metamorph and doing the 3-D reconstruction. When we save a z-series using
export, we can bring the file into Meta, and reconstruct beautifully, but
the image-parameter file is are not included - the file is kind of "dumb".
We are used to having that info still attached - so we can go back, look at
the settings, voxel-size etc. SO, we have tried just "save", and we get a
Leica file with all the info, and then each z-section file, by channel. We
try to import the z-sections into Meta using "build stack" then "numbered
names", but the resulting image is always garbled. How do you do this,
without going through and separating each channel? I have tried the Image
analysis separation tool, but no luck there either. I know there must be a
way - all this was the default "save" result using TCSNT, I can't believe
the new software doesn't do that much. Again, I would really appreciate the
help - thanks-
Betsey
************************************************************************
Betsey Pitts
Research Associate/Microscopist
The Center for Biofilm Engineering
http://www.erc.montana.edu
366 EPS Building, Montana State University
Bozeman, MT 59717
[log in to unmask]
Ph (406) 994-7813
Fax (406) 994-6098
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-----Original Message-----
From: James Chalcroft [mailto:[log in to unmask]]
Sent: Monday, October 21, 2002 6:22 AM
To: [log in to unmask]
Subject: Re: z series
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Dear John,
We continue to use the older Leica NT (Excel-based) software here. If you
are also still using this version you can save the interesting part of your
focus series by:
1) entering "gallery" mode, then selecting (with mouse and simultaneously
pressed Ctrl key) each image of the part-series that you want; (each
selected image will have its frame boldly outlined).
2) use "File/save selected" to store the file. This writes out the part
series as "scanner file format", i.e. multiple-page TIFF.
3) open this newly-written file and you will now see a new gallery
containing only the images you selected.
This method is very useful for clearing away dirt and other bright but
unwanted things from uninteresting planes above and below the planes of
interest which would otherwise dirty-up the items of interest, especially
when projected in maximum intensity "proj" mode. The method might also work
with the later SP2 software, but I haven't tried it out there. Best
Jim
-----Original Message-----
From: John Runions [mailto:[log in to unmask]]
Sent: Friday, October 18, 2002 11:30 AM
To: [log in to unmask]
Subject: Re: z series
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Hi all,
Just a general comment/question on this topic. It seems to me that years
ago I could simply go to the gallery of images within a Zeiss Z-series,
select the portion of the series that I wanted to project and then project
it. Is this true? The reason I ask is that that would seem to me to be a
very useful feature. I now use Leica confocal software and although I have
mentioned this idea to several different engineers and sales people they
have always just looked at me funnily (mind you, a lot of people do
that) as
if this had never occurred to them. What I do now is manipulation of
the
image stack as has been described for Biorad stacks. Is there a simpler way
to do this with Leica software?
John.
-------
C. John Runions, Ph. D.
Department of Plant Sciences
University of Cambridge
Downing St.
Cambridge UK
CB2 3EA
email: [log in to unmask]
phone: (01223) 766 545
http://www.plantsci.cam.ac.uk/Haseloff/JohnRunions/Home.html
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