Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi-
Just wondering if any of you have an idea about the pH dependence of
fluo-3 fluorescence.  I know the Kd goes down as a result of getting
closer to the pKa of the chelator (i.e. the on rate increases).
Therefore, the fluorescence due to Calcium binding at any given
concentration will be lower at a lower pH, presumably.  However, what
I'm searching for is whether protonating one of the chelating groups
will cause an increase in fluorescence on its own.  I'm waiting on an
article about this from ILL, but thought I'd try you all too...
Thanks-
Sarah

---------------------------------------------------------------
Sarah Locknar, PhD
Director, Neuroscience COBRE Imaging Core
University of Vermont
802-656-0413
---------------------------------------------------------------